Plant flow cytometry

From: John Mottley (J.Mottley@uel.ac.uk)
Date: Wed Dec 11 1996 - 05:14:23 EST 

Hi,

I have just joined the list. I'm particularly interested in knowing 
other workers interested in flow cytometry of plant material 
(probably an enthusiastic minority!). I am currently using a Partec CA-III 
to determine DNA amounts in roses.
 
This is a particularly difficult species viz. phenolics. Does anyone 
have experience with contolling phenolics in plant tissue as we have 
found they cause a great deal of fluorescence quenching, at least 
with DAPI staining.

I am also interested to know if Hoechst dyes have a different 
permeability to intact plant cells than does DAPI. 

Thanks

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