Re: mRNA detection using flow

From: l.mclean@auckland.ac.nz
Date: Wed Dec 11 1996 - 13:49:06 EST


Hi Aleta,

For a method for flow detection of cytokine mRNA, try   Morvan, PY et al (1994) 
"In situ hybridisation and cytofluometric analysis of cytokine mRNA during in vitro 
activation of human T cells," European Cytokine Network 5 (5): 469- 480.

I'm interested in this too, but probably won't have a chance to try it for a couple of 
months - please let me know how you get on! If it worked well it would be a lot 
cheaper (and more versatile) than using MoAb's.

Lachy McLean

         -------------  *********  --------------
Lachy McLean 
Senior Lecturer in Clinical Immunology
Department of Molecular Medicine, University of Auckland
Private Bag 92019, Auckland 1, New Zealand
Tel: 64-9-373-7599 ext 6170    Fax 64-9-373-7492
EMail: l.mclean@auckland.ac.nz


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