From: Albert D Donnenberg, PhD (donnal@novell1.dept-med.pitt.edu)
Date: Wed Nov 20 1996 - 13:04:25 EST
Flow'ers We have started a project looking at intracellular proteins (p21, p27) in combination with DNA content. Proteins are measured by indirect fluorescence (FITC GAM) and DNA with PI. Although our early data is interpretable, we have noticed that the PI cvs vary and are not always as sharp as we would like. What factors most affect the tightness of DNA peaks measured with PI? Your help is most appreciated, ******************************* Albert D. Donnenberg, PhD Associate Professor of Medicine University of Pittsburgh W1056 BST 200 Lothrop St. Pittsburgh, PA 15213 (412) 624-9596 *******************************
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