From: Thomas Delohery (t-delohery@ski.mskcc.org)
Date: Mon Nov 18 1996 - 02:08:50 EST
>Recent discussion about the 4th color on FACSCalibur prompts me to point
>(again)
>that, as Angela Schultz points out, there are many options. These are based on
>the recently developed Cy7 tandems of both PE and APC. While Tom Frey is
>correct that Cy7 is not efficiently excited at 635 nm, my guess is that Tom
>Delohery was talking about the Cy7 tandems.
I was speaking about Cy7 tandems. I was also under the impression that the
'Calibur measured 4-colors with 3 PMTs, where FL3/FL4 were collected in one PMT.
I have been informed by Tom Frey that BD has discontinued that design and now
uses separate PMTs for each fluorescence.
td
Look out Mama, there's a white boat coming up the river.
Got a big red beacon, and a flag, and a man on the rail.
I think you'd better call John,
'cause it don't look like they're here to deliver......
the mail.
- NY (NOT New York)
--
==============================================================================
Thomas Delohery | Internet: t-delohery@ski.mskcc.org
Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729
Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019
1275 York Ave. Box 98 |
New York, NY 10021 |
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