From: Jeff Carrell (Jeff_Carrell@hgsi.com)
Date: Thu Nov 14 1996 - 16:29:15 EST
We are trying to generate absolute counts for different cell types in the peripheral blood of mice. What are your preferred methods? I understand that one common practice is to multiply the WBC (from an automated cell counter such as a Coulter Counter) by the percent staining (from FACS). However, we are questioning the validity of this approach because of the different lysing methods used for the two types of instruments. Some non- erythrocytes may be lysed in different proportions with each method. Is multiplying this WBC count by a relative percentage accurate? Of course, these instruments were designed for human blood, so trying to adapt procedures for mice is a challenge...but it shouldn't be impossible. Any thoughts would be greatly appreciated. Jeff Carrell Human Genome Sciences
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