From: Anya Shcherbina (shcherbina@cbr.med.harvard.edu)
Date: Tue Oct 29 1996 - 22:05:05 EST
Need help with staining of human neutrophils for fluorescent microscopy
(cytoskeletal proteins).My protocol works fine for platelets and lymphocytes,
but not for neutrophils. After fixing with 2% paraformaldehyde and
permeabilizing with 0.2-05 % Triton cells are negative even for actin. I tried
another protocol: fixing with 1% glutaraldehyde, permeb. with 0.1% NP40 -
stains fine for actin, but negative control is as bright as specific staining.
My E-mail adress:shcherbina@cbr.medharvard.edu
Anya Shcherbina
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