From: Bert.Schutte@MOLCELB.UNIMAAS.NL
Date: Tue Oct 22 1996 - 12:43:18 EST
Aki Hoji raised the question whether Annexin V labeling could detect apoptosis before actual DNA fragmentation. I have heard the same but I am not aware of any publication on this issue as yet. In reply to Piet van Erp's question, I would say that there are some possible pittfalls of the Annexin V labeling due to the cell isolation procedure. As mentioned in the article by van Engeland et al. (Cytometry 24, 131-139, 1996) use of trypsin to prepare single cell suspensions might induce artificial PS exposure. We don't have experience with cells isolated from solid tissues and tumors but I could imagine that similar problems might occur. Bert Schutte Department of Molecular Cell Biology & Genetics University of Maastricht The Netherlands
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