Tunnel vs Annexin V labelling

From: Aki Hoji (akhst7+@pitt.edu)
Date: Thu Oct 17 1996 - 20:57:30 EST


Hi All,  

I saw a few postings on tunnel staining and Annexin V staining for 
detection of cells undergoing apoptosis.  I was told that Annexin V 
labeling actully could detect apoptosis before actual DNA fragmentation 
(detected by Tunnel assay) occured.  Do this make Annexin V labeling 
better method for detecting apoptosis ?  Obviously, Annexin V staining is 
much easier, thus more convenient than Tunnel, but I wonder if Annexin V 
expression is better marker than DNA fragmentaion for the apoptotic 
cells.  

Aki Hoji (akhst7+@pitt.edu)
University of Pittsburgh


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