From: Derek Davies (davies@icrf.icnet.uk)
Date: Wed Sep 25 1996 - 07:46:20 EST
There has been some discussion on this list about the third colour to be used in a 4 colour surface-labelling experiment. If one is using a He-Ne as the second laser presumably there could be problems with using a tandem dye where the second half of the tandem is Cy5 or APC or whatever that will be excited by some degree by the second laser. Using a non-tandem dye such as BD'd PerCP can get round that problem, but it is well-documented that PerCP is photobleached at high laser powers that are used on sorters. The reason I am writing all this is that today I have received some literature about a dye called ALPHA-RED which comes from Exalpha Laboratories in the States (Through SeroTec here in the UK). I have been told that this is not a tandem dye and that it is excited at 488nm and emits at 670nm so it is obviously a possible alternative to PerCP. Has anybody out there heard of this dye or used it? Any comments? Derek Derek Davies FACS Laboratory, Imperial Cancer Research Fund, London, UK http://www.icnet.uk/axp/facs/davies/index.html Simon wrote: >>I too would like to be illuminated as to which dyes can be used for four >>colour work with the Calibur, are we really stuck with PerCP as the only >>third colour? >>Simon >>Simon Monard >>FACS Lab Manager >>Aaron Diamond Center for AIDS Research
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