From: H.-Joachim Schuberth (jschub@Immunologie.tiho-hannover.de)
Date: Fri Sep 13 1996 - 07:25:54 EST
Dear flowers, recently a thread dealt with fluorochromes for cell tracking experiments. We tried 5(6) CFDA obtained by Sigma, dissolved it in DMSO, diluted it further in PBS and store aliquots at -20oC Problems: (1) bovine and canine Cells (lymphocytes after ficoll isopaque separation or leukocytes after hypotonic blood lysis) tend to aggregate very seriously when labelled in PBS w/o Ca2+, Mg2+ WHY ? (2) not only viable, also (sometimes) dead cells are labelled ! WHY? (3) cells loose their fluorescence very quickly (2-4 days after labelling) WHY Obviously, I have made some seroius mistakes could someone please enlighten me ? thanks in advance Hans-Joachim Schuberth Immunology Unit Veterinary School Bischofsholer Damm 15 D-30173 Hannover Germany
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:31:28 EST
