From: Ress, S, Stan, Dr (srress@liver.uct.ac.za)
Date: Sun Sep 08 1996 - 16:58:27 EST
Hi all, We would like to stain human PBL for simultaneous dual color surface markers, and IFN gamma intracellular expression, using a coulter epics profile 2. We would like to quantitate IFN -G production by memory T cells and CD4/8 subsets in this way. Most of the commercial mca to surface markers will be FITC/PE combinations, does anyone know of any available IFN-gamma MCA conjugated to a third fluorochrome which could be used in these experiments? I understand that for I.C. work PE should be used, not FITC, but this won't work as PE-conjugated MCA will be used for surface marker as indicated. Any advice? Thanks, Stan. Stan Ress Clinical Immunology laboratory Dept of Medicine University of Cape Town Cape Town, South Africa e-mail: sress@uctgsh1.uct.ac.za Phone: Intern + 2721-4066201 FAX : Intern + 2721-4486815
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