From: @rmslab.rockefeller.edu
Date: Fri Jul 19 1996 - 09:24:23 EST
hello to all on the list an investigator here has been studying the effect of various peptides on er negative cells (monocytes and dendritic cells). our question is whether using ionomycin (as our positive control/at various concentrations) should the ratio of violet to blue return to near baseline after the influx of calcium? we are loading with indo-1 and are using an argon ion laser tuned to UV. we get increases in ratio of violet to blue of about 4:1 after stimulation but this stays high even for 10 minutes. this is my first time doing calcium flux experiments and i would be very grateful to hear from anyone with some comments. thank you very much. frank isdell flow cytometry the rockefeller university 1230 york ave ny 10021 tel 212-327-7657 fax 212-327-8875
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