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From: Steven Weintraub (weintrau@visar.wustl.edu)
Date: Tue Jul 02 1996 - 15:20:16 EST

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We are trying to use the enhanced green fluorescent protein (GFP) to
identify transiently transfected cells for cell cycle studies. We want to
quantitate DNA in the cells with propidium iodide and assess for new DNA
sysnthesis with BrDU. Several procedures that we have used to permeabilize
the cells to allow for PI or BrDU antibody uptake inactivate the GFP. Has
anyone worked out a method for using GFP in this manner?


  Steven Weintraub, M.D.
  Departments of Internal Medicine and
  Cell Biology and Physiology,
  Washington University School of Medicine
  at Washington University Medical Center
  Campus Box 8052, 660 S. Euclid Ave.
  St. Louis, MO 63110-1093
  (314) 362-8964  FAX:  (314) 362-8987

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