From: C.Reutelingsperger@BIOCH.RULIMBURG.NL
Date: Wed May 01 1996 - 06:33:24 EST
Hi Kathy, Currently several companies supply Annexin V-FITC. The product quality may vary with the source. We have used the Annexin V-FITC probe from R&D and from NeXins Research. There is a clear difference between the two products. The Annexin V-FITC probe of NeXins is much brighter then the R&D's and distinguishes subpopulations of cells with intermediate levels of PS exposure. These are likely early apoptotic cells, which have not yet fragmented DNA. We have, however, not yet combined directly a TUNEL assay with the Annexin V-assay. Indications that early apoptotic cells expose PS prior to DNA fragmentation come from studies comparing PS exposure and nuclear morphology by flow cytometry, light- and electron microscopy. Chris Reutelingsperger, Ph.D. Department of Biochemistry University of Limburg Maastricht, the Netherlands
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