From: Albert D Donnenberg, PhD (donnal@novell1.dept-med.pitt.edu)
Date: Wed Mar 27 1996 - 20:07:05 EST
Gerry- While we are beating isotype controls to death, I should mention one case where we find them helpful: In 3-color studies we often use one color as a gate (e.g. CD34 plus side scatter) and the other 2 colors to define subpopulations (e.g. HLA-DR and CD38). Since the gated population has very different nonspecific fluorescence than the ungated cells, and since the subpopulations are not always as clear as one would like, a CD34- isotype-isotype control is very useful. Note that the usual isotype-isotype-isotype control would be of no value in this situation since it tells you nothing about nonspecificic binding in the population of interest (in this example CD34+ cells). @dd Albert D. Donnenberg, Ph.D. University of Pittsburgh Voice: (412) 624-9596 Fax: (412) 624-9624
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