Re: Quantifying LIVE CELLS

From: AAllen1216@aol.com
Date: Fri Mar 15 1996 - 11:45:11 EST

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 >REGARDING           Quantifying LIVE CELLS

>I am planning to run a survival bioassay were then I can quantitate LIVE
cells only >using the flowcytometer.  
>I called Molecular Probes and they suggested "SYTOX" which stains dead cells
 >only (but will not cross the membrane of live cells).  Have anyone used
this stain >before?  Advantages or Disadvantages?
>or is there a live cell staining? Suggestions......

Have you tried using a combination of FDA & PI?  FDA will stain live cells
only and PI will stain dead cells.  If you do a two color staining, you can
get an accurate count of live cells.

AAllen1216@1216.com

Next message: Dan Smith: "PI vs. 7-AAD Nuclear Staining"
Previous message: Woods-Cook Kathie MSM PH223: "RE: Bernoulli disks"
Maybe in reply to: Antony Bakke: "Quantifying LIVE CELLS"
Next in thread: Dave Coder: "Re: Quantifying LIVE CELLS"
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