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From: Joern Schmitz (jschmitz@bih.harvard.edu)
Date: Thu Mar 07 1996 - 12:53:37 EST

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Hello colleagues:  Does anyone have any experience with mRNA analysis of 
sorted cells?  We are wondering if it is it possible to obtain relatively 
intact mRNA after hours of cell preparation and sorting.  In other words, 
is the procedure too long, causing extensive mRNA degradation, to allow 
this sort of analysis?  We're anxiously awaiting replies and/or help 
with this problem.  
Thanks in advance for your help!
Joern Schmitz, MD

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