From: O. Joseph Trask, Jr. (otrask@postbox.acs.ohio-state.edu)
Date: Wed Feb 21 1996 - 20:05:35 EST
Greetings Flow People- Is anybody out there doing five color surface immunophenotyping by flow cytometry? If so what fluorochromes are you using? My strategy is FITC, PE, Red613, Cy-X, and a UV excitable dye such as Casade Blue or AMCA. How good are these dyes (Casade Blue & AMCA) ? Are there any other dyes available for a fifth color - my cytometer is equipped with 488, 633, and multi-line UV laser. APC is out of the question, since Red613 (PE-Texas Red Tandem) dyes are weakly excited by 633 line . Any suggestions will be useful. Thanks in advance. Regards, O. Joseph Trask, Jr. The Ohio State University Comprehensive Cancer Center 223 Wiseman Hall, 400 West 12th Avenue Columbus, Ohio 43210 Tel: 614/292-FLOW(3569) Fax: 614/292-7335 E-Mail: cytometry@osu.edu
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