Flow cytometry vs. Immunoblotting and Enhanced Chemiluminesence

From: Alan Salkind (nepsy@umsmed.edu)
Date: Wed Feb 14 1996 - 11:57:23 EST

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What are the relative attributes of detecting antigens (specifically those
that are intracellular) by flow cytometry vs. ECL?  In our system, we've
found immunoblotting and ECL to be much more sensitive.  Does anyone know
the limits of antigen detection of flow vs. ECL?  


Thanks in advance for your comments and ideas.

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