Clastogenicity by CV

From: /R=HERLVX/R=AM/U=ELSTEIN/FFN=ELSTEIN/@mr.rtpnc.epa.gov
Date: Thu Feb 01 1996 - 14:25:04 EST

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Fellow Flowists:

I would appreciate any opinions regarding the validity of an assay that
purports to detect the clastogenic potential of a toxicant as a widening of
the CV (presumably, CV half max) of the G1 peak of exposed CHO cells.  

In one paper I've been given, the authors incubated exposed cells in a
cocktail containing 0.05 mg/ml PI, 0.1% Triton X-100, 0.1% sodium citrate,
and 7 units/ml RNase for 40 min in the refrigerator before analyzing on a
Coulter EPICS 750.  

Many associated aspects of the paper make the data suspect (e.g., variable
staining, insufficient details on method, no verification by another
method, no standard deviations or confidence limits [just statistical
significance]). Nonetheless, taking the assay by itself (but in the context
of the numerous factors during preparation and analysis that can influence
CV), are people comfortable with this technique as an index of toxicity? 

Ken Elstein

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