From: kukuruga%kasle1.dnet.wayne.edu@rocdec.roc.wayne.edu
Date: Fri Jan 26 1996 - 13:41:56 EST
Reply to Dr. Farkas...the easiest answer is that these tissues have been fixed with formalin prior to raraffin embedding. This develops crosslinks which limits accessibility to the DNA by propidium iodide -- therefore, less dye means less fluorescence, so your peaks will naturally be lower. Several attempts at eliminating this have been reported -- the best of which I was from a recent Cytometry article where the authors (I apologize for not remembering the names...if it's necessary, I'll look it up -- I think it was Overton?) used heating to elinimate the effect.
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