From: /G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Date: Fri Jan 26 1996 - 05:03:00 EST
We used the polarising scatter on the Coulter EPICS Elite
with the argon and the HENE successfully. You just have to
sort out the polarisation planes of your lasers and get the
filters / polarisers adjusted for the detection. The
autoflourescence is easier as it does not need all that fuss
and picks up the same population.
Gerhard.Nebe-von-Caron@urcgb.sprint.com
______________________________ Reply Separator _________________________________
Subject: RE: Eosinophils
Author: p.openshaw@ic.ac.uk at INTERNET
Date: 25/01/96 18:30
In response to Janek Brozek:
> there is any antigen allowing POSITIVE selection and sorting of
> eosinophils... To my knowlege there's none, but ....
>
de Grooth et al (1987) Cytometry 8:539 used rotation of polarised light by
eosinophils to sort PNM and Eos on FALS/side scatter. A student in the lab
tried this with limited success, although the Abbott haematology analysers
apparently use this method routinely. Weil and Chused (1981) Blood 57:1099
used blue autofluorescence. Another similar method was used by Lehnert and
Steinkamp (1986) Cell Biophys 8:201 on rat pleural eos. Others select CD9+
16- granulocytes, which are mostly eos.
We would dearly love a good method, so please let me know what you decide
on.
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