From: Gary Durack (durack@ux1.cso.uiuc.edu)
Date: Thu Jan 25 1996 - 15:06:22 EST
This is a post from a colleague ... Cytometry Experts, We are doing flowcytometric analysis of nuclear DNA content and ploidity determination of paraffin embedded archival pathological lymphoma cases in dogs.We dewax the 30um sections by two changes of xyleneand dehydrate with sequential ethanol concentrations and distilled water.Finally we digest the tissue by pepsin and stain by standard PI staining method. We get nice diploid and aneuploid peaks,however the fluorescence of the deparaffinized samples is lower than in the fresh material. Questions:Is this the expected pattern of paraffin embedded tissue? If yes:what is the explaination? If not:how can we increase PI uptake in archival lymphoid tissues? farkas@ux1.cso.uiuc.edu Amnon Farkas Univeristy of Illinois School of Veterinary Medicine Gary Durack University of Illinois Biotechnology Center Flow Cytometry Facility Voice (217) 244-0559 FAX (217) 244-0466
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