From: Margaret Cooley (m.cooley@arnie.cfi.unsw.EDU.AU)
Date: Wed Jan 24 1996 - 16:32:03 EST
We have been routinely running 4 colour analysis on HIV patients on our XL with FITC, PE, ECD and PerCP, although not with the combinations you suggest. It works very well, with careful compensation, although it can depend somewhat on the "brightness" of your antibodies. Compensating PE out of ECD and vice versa seem to be the worst. We are usually using CD3 PerCP, CD4 or CD8 ECD and using PE and FITC for other markers of interest. If you want more details, the person who does this work is John Zaunders: he doesn't have internet access, but I could pass on messages. Good luck Margaret Margaret A. Cooley, PhD Centre for Immunology St Vincent's Hospital Darlinghurst NSW 2010 AUSTRALIA Ph: 61-2-3617700; Fax: 61-2-3612391.
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