Re: Equine flow cytometry

From: /G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Date: Mon Jan 15 1996 - 05:17:00 EST


          Contact VMRD, Inc.(Veterinary Medical Research and 
          Development
          
          P.OO. Box 502
          NW 115 State Street
          Pullman
          
          WA 99163
          Tel 800/222-8673
              500/334-5815
          Fax 509/332-5356
          
          
          They have CD2, 4, 5, 18 and 44. It is sometimes worthwhile 
          to check the anti humans for crossreactivity. I found the 
          CD14 from DAKO to crossreact with swine and the CD14 from BD 
          to work nicely with rabbit. DAKO also has a nice CD 3 that 
          may work across species but it recognises an intracellular 
          antigen
          
          Gerhard Nebe-v.Caron
          Unilever Research, Colworth Lab.
          Sharnbrook, Beds. GB-MK44 1LQ
          Tel.:+44(0)1234 222066
          Fax.:           222344
          Gerhard.Nebe-von-Caron@urcgb.sprint.com









______________________________ Reply Separator _________________________________
Subject: Equine flow cytometry
Author:  Roger@cpd2.usu.edu at INTERNET
Date:    12/01/96 19:24


To All:
          
          
I have been asked to evaluate equine peripheral blood leukocyte phenotyes 
with flow and was wondering if anyone knows of a source of either 
conjugated or unconjugated antibodies for EqCD3, EqCD4, EqCD8 and a B cell 
antibody (EqCD19?).
          
I also need to evaluate NK cell activity in vitro.  Does anyone know of a 
target cell line that is used for this purpose?
          
Thank You.
          
***********************************************
          
Roger A. Burger         E-mail: Roger@cpd2.usu.edu 
Research Immunologist
Center for Persons with Disabilities 
Utah State University
Logan, UT  84322-6895
Voice: 801-797-2042
FAX:  801-797-2044


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