Toxicology Phenotyping, Monkey, Rat, Dog

From: ABBOTT DAVID L JR (ABBOTT_DAVID_L_JR@Lilly.com)
Date: Wed Jan 10 1996 - 22:23:00 EST


Greetings:  

This probably applys to a small group of reasearchers, and that is why I 
used the awkward subject title. Any information provided will be 
appreciated. 
   
How many sites are performing peripheral blood lymphocyte phenotyping 
for toxicology studies using the rat, mouse, monkey or dog? I am 
interested in the basics (T, B, CD4, CD8, NK). Are the studies performed 
routinely or reactively (based on lymphopenia for example). 

Are you reporting results as relative percentages or absolute counts? 
Has anyone used the instrument from Cytek to obtain absolute counts on a 
FACScan. I am in the middle of a study, and the issue of percentage vs 
absolute counts has been raised, although sample volumes restrict 
absolute count methods for the mouse and rat. 

Regards,

David Abbott
DL.Abbott@Lilly.com    


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