From: kjartan.egeberg@ifk.unit.no
Date: Thu Dec 21 1995 - 05:17:24 EST
Hi, sorting flowers. It is my experience that micro airbubbles in the sheet-fluid can cause problems during sorting, mainly by drifting of the dropformation, thereby reduse the purity and yield during a run. I therefore, with good success, routinously de-gass my sheet-fluid to "no boiling" with a vacumpump and a cold-trap between the pump and sheetfluid (not to destroy the oil-based vacumpump). This is actually the speed-vac system adapted to fit the fluid tank. It might be acceptable to use a standard water-jet pump to create the vacum for de-gassing. But, of course, if there are leakages in the system (O-rings, tubes, etc.), this will cause troubles of unstability. These problems should be easy to evaluate. Have a merry X-mas and a happy new flowyear ! Kjartan Egeberg Inst. of Canser Research and Mol. Biol. University in Trondheim N-7005 Trondheim Norway T.phone: (47) 73 59 86 70 T.fax: (47) 73 59 88 01 E.mail: Kjartan.Egeberg@ifk.unit.no
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