From: kelley@wh.bayer.com
Date: Thu Dec 21 1995 - 10:25:25 EST
> Hello > I was wondering if anyone has experience loading COS-7 cells with > Indo for calcium mobilization analysis. We've been having a tough > time with this. The Indo seems to be ok, since we've loaded other cells ...etc. > Peter Lopez I asked a similar question recently regarding CHO cells and one of the suggestions from the list was to use verapamil. We tried loading in 4uM INDO-1, 30uM verapamil for 1 hour (including the pluronic) and it worked great. The fluorescence we got was an order of magnitude better than loading in the absence of verapamil. I would suggest this as a first try to see if the COS cells are actively pumping out the dye as it is being cleaved. Good luck! Keith ********************************************************************** * Keith A. Kelley kelley@wh.bayer.com * Institute for Research Technologies * "No tree is too big Bayer Corporation * for a short dog to 400 Morgan Lane Ph 203-931-5303 * lift his leg on" West Haven CT 06516 FAX 203-937-5467 * * Texas Bix Bender **********************************************************************
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