apoptosis by flow

From: hwortis_sup@OPAL.TUFTS.EDU
Date: Wed Dec 20 1995 - 23:39:58 EST

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Is this a problem that other people have solved?
	We have been using flow to analyze apoptosis in lymphocytes.  We 
have been using P.I. staining and using either ethanol or hypotonic lysis 
to obtain nuclei and stained nuclear fragments.  The problem is whether 
to use FSC/SSC gates.  If the answer is yes, which particles do you omit 
from the gate?
	It seems that there is a real problem of analysis.  Each 
apoptotic nucleus may form several fragments.  Therefore there 
is no defined relationship between the number of fragments and the number of 
apoptotic cells.  Do other flowers recognize this as a problem?  If so 
how do you solve it?

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