Disappearing monocytes

From: Dave Lundy (dlundy@inforamp.net)
Date: Wed Dec 27 1995 - 09:31:36 EST

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I am posting this on behalf of a colleague who is not on the 'net'.    He is
running an Elite ESP, equipped with air-cooled lasers, and the 3x 100-micron
flow cell ("Sort-Sense").   The (prepped whole blood) samples run and
accumulate normally, until he increases the sample rate.    Once the rate is
above 1000-2000 per second, the monos "disappear" !   Reducing the rate
again brings the monos back into the accumulation.   We think this might be
due to the monos binding to the sample tubing, and/or other surfaces.   Is
there a way to prevent this?
Regards,

Dave Lundy.
(Coulter Canada Ltd.)
dlundy@inforamp.net
Work:  Coulter Canada Ltd., 905 Century Drive, Burlington, Ontario  L7L 5J8
TEL:  905-452-9187     FAX: 905-333-3787

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