From: Dennis Broud 301-594-6259 FAX 301-443-9292 (BROUDD@fdacd.bitnet)
Date: Thu Dec 21 1995 - 10:28:42 EST
I would occasionally see them with the LysisII setup as well. I suspect that my problems are more along the line of "air bubbles" rather than crystals, as all my staining and dilution buffers, as well as tissue culture media are filtered through 0.2 micron filters before use. It is however somewhat strange or at least serendipitous that these "air bubbles" have the same forward scatter range as human lymphocytes. The BD service man seems to think that it looks more like an intermitant communications problem between the cytometer and the computer.
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