Indo loading

From: PLOPEZ@sorter.dfci.harvard.edu
Date: Wed Dec 20 1995 - 15:15:44 EST

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 Hello

  I was wondering if anyone has experience loading COS-7 cells with
 Indo for calcium mobilization analysis. We've been having a tough
 time with this. The Indo seems to be ok, since we've loaded other cells
 nicely with the same stock.  We are using the standard protocols for
 loading and have varied time from 1/2 to 1 hour, also varied the Indo
 conc., also tried loading the cells 1/2 hour after they've been taken off
 the dish (to let them calm down ).
  We haven't tried the Pluronic trick yet, and haven't tried loading in
 the flask before lifting the cells. Any suggestions.
   Oh, have a safe and happy holiday season out there!

 Peter Lopez

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