From: Dennis Broud 301-594-6259 FAX 301-443-9292 (BROUDD@fdacd.bitnet)
Date: Fri Dec 15 1995 - 18:00:06 EST
I too have seen this curious little episodic "burp". As I work with primarilarly PBMC-Scid mice, I get a nice little "straight line" right at the bottom of the FSC/SSC (x-axis/y-axis) plot directly below the major lymphocyte population on my FACSORT. I've seen it so often that I can pretty much tell where its going to be, so I set my aquisition gate for everything above it, and ignore it. Essentially I set a normal forward scatter threshold and then use a nearly horizontal line across my FSC/SSC plot to exclude the machine aberation. I don't always see the aggravating little "burp", but I've worked my way around it. Now that you've mentioned it though it doesn't make any sense that it should be where it is. If it was clumps it ought to be at the other end of the scale. I never have looked at the voltage during these blips, but will do so in the future. Who at BD have you been talking to? I'd like to find out what they think.
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