From: Roger Burger (Roger@cpd2.usu.edu)
Date: Mon Dec 11 1995 - 14:21:03 EST
I have recently been asked to work with some peripheral blood cells from a bone marrow transplant recipient with CML. I would like to be able to isolate the leukemic cells from the other leukocytes and was wondering if someone could tell me if these cells have a specific phenotype I can look for. I suspect that these cells are CD34+ DR+ and at some point following blast crisis they express CD19 but I am not to sure on this and hope that one of you working in a clinical stetting can help me with this. I also want to culture these cells in vitro and have a 1993 paper by Morel, et al, suggesting the use of sCD23 and IL-7 in culture. I plan to use the CML cells as stimulators for CTL and eventually targets for measuring CTL reponse. I will need to keep them in culture for several weeks. Again any ideas on this topic would be greatly appreciated. Thanks *********************************************** Arly A. Helm, nutritionist, lactation consultant email: arly@cc.usu.edu Roger A. Burger E-mail: Roger@cpd2.usu.edu Research Immunologist Center for Persons with Disabilities Utah State University Logan, UT 84322-6800 Voice: 801-797-2042 FAX: 801-797-2044
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