synchrony

From: Joanne Clark (joanneC@qimr.edu.au)
Date: Mon Dec 11 1995 - 21:18:38 EST

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I agree with Thomas Delohery.  Drug induced cell cycle blocks are very
informative especially with regard to proteins in G2/M phase (normally a
very low population) when done properly.  In our lab we use a double
thymidine block synchrony, and our cells (this technique is mostly used on
HeLa cells) block and release very well.  We also have used both
Hoechst33342 and aphidocholin on occasion on a variety of different cells
with success (ie fairly synchronous release) gaining valuable data on the
cell cycle.  Anybody not having success with drug blocks needs to try
different concentrations of drugs, and different times of incubation.

Joanne Clark

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