From: Kai Toellner (TOELLNKM@novell1.bham.ac.uk)
Date: Fri Dec 01 1995 - 04:04:13 EST
> Begin forwarded message: > > Date: Wed, 29 Nov 1995 08:52:33 +0800 > X-Sender: nmilkh@leonis.nus.sg > X-Mailer: Windows Eudora Version 1.4.4 > To: cyto-inbox > From: nmilkh@leonis.nus.sg (KH Leong) > Subject: Re: 4 color flow > Cc: "David L. Haviland, Ph.D." <HAVILAND@kids.wustl.edu> > > ...Although we can detect PerCP on the EPICS, we have not been able to do so > on our FACStarPLUS! We are still waiting to hear from BD...and meanwhile we > are trying to figure out the sets of filters and dichroic mirrors for the > FACStar+. Maybe you or someone else out there could help? > > ------ > > PerCP is very photolabile. 50mW of 488nm light is enough to kill the > fluorochrome in my experience. Those who have reported that it works have > used either air-cooled argon lasers or N.D. filters to decrease the power > output of a water-cooled laser. A 670 band pass filter will work just fine > for detection of PerCP fluorescence. > > Dave Coder > dcoder@u.washington.edu > Dyes which work very well with the FACStarPlus are RED613 and RED670 from Gibco. Kai Toellner ---------------------------------------------------------------------- Dr. Kai-Michael Toellner Department of Immunology The Medical School Tel.: (44) 121 414 6970 University of Birmingham FAX.: (44) 121 414 3599 Birmingham B15 2TT, UK E-mail: K.M.Toellner@bham.ac.uk
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:30:39 EST
