From: Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
Date: Tue Nov 28 1995 - 13:18:32 EST
Hello Flowers, For those of you following Clevenger-type methods for staining cells for DNA plus an intracellular antigen: after p-formaldehyde fixation and treatment with 0.1% Triton-X, do you leave detergent in the washing and staining solutions for all subsequent steps? Published protocols seem rather ambiguous on this point. We want to cut down on non-specific staining, but suspect that the triton-X will interfere with our specific staining as well. Thanks for your help. Alice Givan Englert Cell Analysis Laboratory Dartmouth Medical School Lebanon, NH 03756, USA tel: 603-650-7907 fax: 603-650-6130
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