Re: FACScan cleanup - Washing solutions ???

From: /G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Date: Tue Oct 24 1995 - 04:14:00 EST


          We use a 10% Domestos solution made up with distilled water 
          and sterilefiltered. This we keep for up to 3 month in the 
          dark at room temperature without problems. Domestos contains 
          15% hypochlorit (in the UK market). It forms a precipitate 
          with (hard) tab water wich you would not want in the FCM.
          
          Gerhard Nebe-v.Caron
          Unilever Research, Colworth Laboratory Sharnbrook, 
          Bedfordshire
          GB - MK44 1LQ
          Tel:    +44(0)1234-222066
          FAX:    +44(0)1234-222344
          E.mail: gerhard.nebe-von-caron@urcgb.sprint.com
          
          


______________________________ Reply Separator _________________________________
Subject: FACScan cleanup - Washing solutions ???
Author:  mhaury@pasteur.fr at INTERNET
Date:    23/10/95 22:34


Hello..
          
I'm just interested in the current washing - cleanup solution that people 
use for cleaning their machines. Our FACScans systematically get clogged up 
because the hypochlorite solution (supposedly 1 %) forms cristals in the 
stock solution.. the only solution is to prepare fresh every day (which I'm 
too lazy to do...).
          
Is there anyother detergent/solution that people use to cleanup that 
doesn't have this problem ??
          
Thanks a lot,
          
Matthias
______________________________________________________________ 
Matthias Haury                      _/_/_/    _/_/_/    _/_/_/ 
Flowcytometry - Immunology           _/     _/    _/  _/    _/ 
Institut Pasteur Paris              _/     _/_/_/    _/_/_/ 
Email: mhaury@pasteur.fr           _/     _/        _/
Tel:   33 (1) 40 61 31 29       _/_/_/   _/        _/ 
Fax:   33 (1) 45 68 86 39       INSTITUT PASTEUR   PARIS
______________________________________________________________


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