From: /G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Date: Tue Oct 24 1995 - 04:14:00 EST
That reminds me of good old Burghard Bohn from Heidelberg,
who introduced the idea of "receptor-ligand" binding to the
field of flow cytometry in the late 70's. He used the
insulin receptor as a model for endicronological studies
possible by FCM. They simply labelled it with FITC. I have
only got his article "Flow Cytometry: a novel approach tfor
the quantitative analysis of receptor-ligand interactions on
surfaces of living cells", Molecular and Cellular
Endicronology, 20 (1980) 1-15. I assume he published a few
more papers on that.
Gerhard Nebe-v.Caron
Unilever Research, Colworth Laboratory Sharnbrook,
Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
E.mail: gerhard.nebe-von-caron@urcgb.sprint.com
______________________________ Reply Separator _________________________________
Subject: Fluorescent insulin
Author: Dean.Hewish@mel.dbe.csiro.au at INTERNET
Date: 23/10/95 21:23
A colleague asked me to enquire about fluorescently labelled insulin.
Any fluorochrome would do.
Has anyone used such a reagent for receptor binding studies on insulin
responsive cells (not necessarily by flow)?
If so, did it show reasonable binding and how was it labelled?
Does anyone know of a commercial supply, or do you need to make your own?
Thanks,
Dean R. Hewish, Cell Biologist & Flow Cytometrist. ( and Amateur Malacologist)
CSIRO Biomolecular Engineering, 343 Royal Parade, Parkville, 3052,
Victoria Australia.
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