third colour indirect labeling

From: /G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Date: Tue Oct 17 1995 - 04:28:00 EST


          Hi everybody
          
          My question was actually regarding anti mouse immunoglobulin 
          antibodies conjugated to a third colour, not anti mouse 
          antibodies. Pharmingen do only some of their isotype 
          controls in cy-chrome. Using an epics-xl I actually want to 
          look at skin derived cells which have a high 
          autofluorescence. With the green autofluorescence I can 
          comfortably compensate the red autofluorescence down in the 
          first decade whilst I do not feel to good about doing it the 
          other way round and I would like to avoid having to use the 
          HENE on the epics elite.
          Thanks to all who responded so far
          
          Gerhard Nebe-v.Caron
          Unilever Research, Colworth Laboratory Sharnbrook, 
          Bedfordshire
          GB - MK44 1LQ
          Tel:    +44(0)1234-222066
          FAX:    +44(0)1234-222344
          E.mail: gerhard.nebe-von-caron@urcgb.sprint.com
          
          


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