Re: 3rd colour indirect staining

From: Julie Auger (jauger@flowcity.bsd.uchicago.edu)
Date: Fri Oct 13 1995 - 15:05:04 EST


>          I did send this question on the 29 of September but could 
>          not find it on the internet database in purdue, so it 
>          probably got lost in outer space.
>          
>          Does anyone know a source for anti mouse reagents direct 
>          labelled with a 3rd colour 488nm excitable or has a good 
>          idea to quench the autofluorescence of squames?
>          
>          Thanks in advance
>          
>          
>          Gerhard Nebe-v.Caron                                  | | 
>          Unilever Research, Colworth Laboratory                | | 
>          Sharnbrook, Bedfordshire                              |o|
>          GB - MK44 1LQ                                         
>          Tel:    +44(0)1234-222066                            /   \ 
>          FAX:    +44(0)1234-222344                           /     \  
>          E.mail: gerhard.nebe-von-caron@urcgb.sprint.com    /       \
>          
>
Pharmingen sell decent reagents coupled to Cy-Chrome ( a tandem conjugate of
PE-Cy-5, I think) to many mouse surface antigens.  The UK distributer listed
in their catalog is Cambridge BioScience, 
                 25 Signet Court
                 Newmarket Road
                Cambridge CB5 8LA
                tel (0223) 316855
                fax (0223) 60732

Julie
********************************************************************************
Julie A. Auger                                        voice:  312-702-9212 lab
Director, Flow Cytometry Facility                       312-702-9261 office
University of Chicago                                 fax:  312-702-3701
5841 S. Maryland Ave. MC-1089                                         
Chicago, IL  60637           e-mail:   jauger@flowcity.bsd.uchicago.edu
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