From: David Galbraith (dgalbrai@ag.Arizona.EDU)
Date: Thu Oct 12 1995 - 18:39:41 EST
I guess the plant folks would like an automatic histogram classification program. Sometimes there are as many as ten (count 'em!) peaks per sample. I think it would be quite tedious to set markers etc. around all of these, espp. if there were multiple samples (i.e. fields of nightmares!). Plus we do need to get rid of noise (a steep exponential (log?) dropping from channels 1 upwards). How many peaks do megakaryocytes show? David P.S. Don't mention fire unless you can abide being flamed! :-) At 11:41 AM 10/12/95 -0400, you wrote: > >I've done extensive work on megakaryocyte ploidy, which will give, I suspect, >similar results to your corny project. A question: is ther some reason you >cannnot use straight histogram analysis techniques (set markers, integrate >the area under the curve, ratio to the total, etc.)? >If you're looking for more...have you tried MODFIT? You can pretty much set >the fitting algorithm to do anything you want. >Also, Bob Murphy has developed several fitting algorithms in his COTFIT >program, if you have access to Consort40 on a MicroVAX (Bob, are you there?). >There seems to be lots of options, unless I'm missing something in your >question. > >P.S. To the folks who "cut" and "weigh"...two questions: 1) what's the c.v. >on your scissors, and 2) have you heard...they've discovered "Fire"!!! > >P.P.S. I apologise for that...I couldn't resist. > > ============== David Galbraith Professor of Plant Sciences University of Arizona 303 Forbes Building Tucson AZ 85721 Phone (520) 621-9153 FAX (520) 621-7186
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