From: David Galbraith (dgalbrai@ag.Arizona.EDU)
Date: Wed Oct 11 1995 - 10:44:46 EST
Gerard: I periodically try to get software companies (or anyone else) interested in writing programs for this purpose -- cell cycle analysis programs capable of dealing with log distributions, multiple peaks, and background subtraction. So far (as far as I am aware) there has been resounding silence. We print out the distributions using something like Sigmaplot, enlarge them, cut out the peaks, and weigh them. This apparently hokey approach is quite accurate and was originally developed for finding the proportion of ribosomes in the various 2-mer, 3-mer,4-mer, etc., peaks found in sucrose gradient analysis of polyribosomes. Hey, maybe there's another application for the software!? Good luck David At 09:34 PM 10/10/95 -0500, you wrote: >Dear colleagues, > >I'm working on DNA endoreduplication in maize nuclei, analyzing the >tissue with a Coulter Epics flow cytometer/MDADSII. I want to calculate >the percent nuclei in each individual C class to see how >endoreduplication progresses as the tissue get older--e.g. how many >nuclei are in the higher C classes over time. Are there any statistical >programs to determine the number of cells/percentage of cells in each >individual C class/peak? > >Thanks in advance, > >Gerard Engelen-Eigles >Dept. Agronomy, university of MN, St. Paul, MN >enge0019@gold.tc.umn.edu > > ============== David Galbraith Professor of Plant Sciences University of Arizona 303 Forbes Building Tucson AZ 85721 Phone (520) 621-9153 FAX (520) 621-7186
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