From: /G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Date: Fri Oct 06 1995 - 11:38:00 EST
As I know you have an Epics Elite, I can confirm having seen
similar problems with lipophilic stains like bis oxonol that
also labels up the tubing. It is a question of dye
concentration, push time and the cleaning process of the
tubing. It is worth therfore checking the backflushing time
and looking at the time versus fluorescence display.
Gerhard Nebe-v.Caron
______________________________ Reply Separator _________________________________
Subject: Weird DAPI observation
Author: hilliard@cb.uga.edu at INTERNET
Date: 05/10/95 21:40
Hi folks,
This one has me scratching my head--we're using DAPI to stain for DNA
content, and our first sample always has a lower fluorescence than the
subsequent samples. We thought at first it was staining variability,
but out of curiosity we re-ran the first tube at the end of the
experiment, and it had gotten brighter, so that all cells had similar
DNA content (as we were expecting). This has been consistent through
the last 3 visits.
Is this some sort of tubing/sheath equilibrium effect? I hadn't
heard of this with DAPI, but we haven't done that much with it. has
anyone seen this before?
Steve
*********************************************************
Steve G. Hilliard Life's too short
Cell Analysis Facility to drink bad beer!
University of Georgia
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