From: /G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Date: Mon Sep 11 1995 - 05:33:00 EST
Try to contact:
Dr. C.T.Nebe
Klinikum Mannheim
University of Heidelberg
Tel.:+49-383/2222
Fax:+49-621-383/3819
I remember when he edited a German book on Clinical Methods
in Flow Cytometry (subsequently published by Schmitz et al)
that they had a routine protocol for heparinised whole blood
called:
Detection of intracellular cytokine
- functional characterisation of immunecompetent cells in
immune defects
- monitoring of immunosuppressive therapy
from a group in Bochum. They used a system of isotype
specific secondary antibodies and triple colour
fluorescence.
Gerhard Nebe-v.Caron
Unilever Research, Colworth Laboratory Sharnbrook,
Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
E.mail: gerhard.nebe-von-caron@urcgb.sprint.com
______________________________ Reply Separator _________________________________
Subject: Intracellular cytokines
Author: DONNAL@novell1.dept-med.pitt.edu at INTERNET
Date: 08/09/95 20:37
Two questions about detection of intracellular cytokines:
1) Does anyone have an opinion concerning the relative merits of
Brefeldin A and monensin for blocking cytokine secretion?
2) Does anyone have information on the availability (commercial or
otherwise) of directly conjugated anti-cytokine antibodies. We have
only been able to come up with one (FITC anti-IL2 from Biosource).
Thanks,
Albert D Donnenberg
University of Pittsburgh
Albert D. Donnenberg, PhD
Associate Professor of Medicine
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