From: allp.attmail.com!APCWESTNET!APCWESTPO!DJS
Date: Tue Aug 15 1995 - 11:56:00 EST
It is said that there are no stupid questions but..... When labeling leukocyte cell surface antigens with the appropriate conjugated antibody, is it best to fix (with paraformaldehyde) the cell suspension: a) before antibody labeling? b) after antibody labeling? c) what concentration of paraformaldehyde (1% final concentration?) and is it dependent upon the d) cell type? e) antigen to which the antibody is specific for? f) monoclonal vs. polyclonal antibody? If that wasn't enough for you, add in the variable of labeling intracellular antigens into the question of timing of fixation. If there is a review of this in the literature, could you point me to it please. Thanks for your time and forthcoming answers. Dan J. Smith San Diego CA
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