From: Alan Bishop (BISHOP@immuno.imvs.sa.gov.au)
Date: Fri Aug 11 1995 - 13:57:55 EST
Its been a while, but 15 years ago (when I was at the Sir william Dunn School of Pathology in Oxford) we used to sort rat fetal liver and bone marrow cells suspended in Percoll to stop them settling and claging the system. There were no observed deterimental effects to the cells (or the Facs II) and we reduced our blockage rate from more than 3 or 4 times a sort to ZERO. +-------------------------------------------------+ | | | Alan Bishop | | Flow Cytometry Laboratory | | Hanson Centre for Cancer Research | | Institute of Medical and Veterinary Science | | PO Box 14 Rundle Mall | | Adelaide SA 5000 | | AUSTRALIA | | | | Voice : 61-8-228 7258 | | Fax : 61-8-232 4092 | | Email : bishop@immuno.imvs.gov.sa.au | | | +-------------------------------------------------+
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