Re: supravital DNA staining

From: /G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Date: Thu Aug 10 1995 - 05:04:00 EST


          Ethidium Bromide might not be too specific for DNA but goes 
          supravital sometimes, or use it as Hydroethidine. A little 
          bit of tween 20 or pluoronic might help the uptake. 
          Otherwise ask molecular probes about their new dyes.
          For UV exited like DNA profiles your chances are pretty 
          slim.


______________________________ Reply Separator _________________________________
Subject: supravital DNA staining
Author:  WHGRLD@cardiff.ac.uk at INTERNET
Date:    09/08/95 17:50


Anyone know of a supravital DNA stain that can be excited with an 
argon laser and which has an emission spectrum similar to 
phycoerythrin.  My objective is to obtain good quality cell cycle 
data in combination with a FITC marker in viable cells.
          
          
Richard Darley
Dept Haematology
UWCM
Cardiff U.K.


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