From: Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
Date: Wed Jul 26 1995 - 14:46:57 EST
We have a scientist who uses DAPI to discriminate between live and dead cells (as with PI). However, Molecular Probes say that DAPI is a cell permeant DNA stain (like the Hoechst dyes) and will stain living cells. Is this just a kinetics difference so that DAPI enters living cells just a bit slower than it enters dead cells? Has anyone used DAPI as an alternative to the Hoechst dyes to stain living cells for ploidy? We want to be able to sort living cells on the basis of their DNA content -- for further culture. Any suggestions? Thanks! Alice Givan Englert Cell Analysis Laboratory Dartmouth Medical School Lebanon, NH 03756, USA tel: 603-650-7907 fax: 603-650-6130
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