From: Matthias Haury (mhaury@pasteur.fr)
Date: Wed Jul 05 1995 - 02:27:20 EST
At 19:14 4/07/95, Mikael Vestberg wrote: >Hello everyone, >Does anyone know how to get instrument settings from a datafile >collected with Cellquest on the Macintosh? It was possible with the HP >system and both should be FCS2 standard, shouldn't they? > >Also, has anyone compared Saponin from Saponaria species and Quillaja >bark and found them to differ (Sigma changed composition but not >catalogue number) when staining for intracellular antigens? We only have >the latter type. > >Best regards, > >Mikael Hi Mikael, as far as I know, there is no way to get the instrument settings from the FCS Datafile, they are not stored in the datafile anymore... Here is a typical header from a Cellquest data file: FCS2.0 256 1591 1792 51791 0 0 \$BYTEORD\4,3,2,1\$DATATYPE\I\$NEXTDATA\0\$SYS\Macintosh System Software 7.1.0\CREATOR\CELLQuest 1.1.1\$TOT\5000\$MODE\L\$PAR\5\$P1N\FSC-H\$P1R\1024\$P1B\16\$P1E\0,0\$P2N\SS C-H\$P2R\1024\$P2B\16\$P2E\4,0\$P3N\FL1-H\$P3R\1024\$P3B\16\$P3E\4,0\$P4N\FL 2-H\$P4R\1024\$P4B\16\$P4E\4,0\$P5N\FL3-H\$P5R\1024\$P5B\16\$P5E\4,0\$CYT\FA CScan\$BTIM\16:52:33\$ETIM\16:52:43\BD$AcqLibVersion\1.1.1\BD$NPAR\5\BD$P1N\ FSC-H\BD$P2N\SSC-H\BD$P3N\FL1-H\BD$P4N\FL2-H\BD$P5N\FL3-H\ and there are not enough instrument settings defined !!!! I don't understand either why BDIS is not following the FCS2 Standard, especially as on the Mac, Data file size is not really a problem (with optical disks being much cheaper than anything on the HP). I hope they are going to reverse this soon !! This doesn't mean that they can't keep a separate settings file as they do now in Cellquest, but at least one could get the settings back from the datafiles as well.. There is several other stupid things in Cellquest (in case the Programmers in BDIS USA are listening to this mailing list ??????? Maybe they'll change this in the next update !!!): 1. Why didn't they integrate the FACSConvert in the program, so that one can read FCS 1.0 and FCS 2.0 files directly ??? Only to ask people to pay a couple of hundred $ extra ??? 2. Why didn't they follow some normal intuition ??? eg. using duplication (copy/paste) to create plot with the same size, allow change of format for each plot (Histo into Dot etc) and so on.. I have a long list of requests.. 3. Why can't one modify the Axis labels after exporting to Claris Works... 4. Why do they sell Quickkeys if it is incompatible with Cellquest (exept of the most stupid macros, everything a bit more complicated crashes Cellquest...). 5.-100. I don't want to go into more details, just suggesting that we as users should try to convince the programmers to take into account our critics !!! Especially as Cellquest is listed for more than 5000 $ here in France !!! I would hope that if we are many to complain about the same things, maybe we can create a wishlist for BDIS (at least until someone comes up with a free program on the Mac that is more suited for scientific use...). Matthias ______________________________________________________________ Dr. Matthias Haury _/_/_/ _/_/_/ _/_/_/ Flowcytometry Laboratory _/ _/ _/ _/ _/ Department of Immunology _/ _/_/_/ _/_/_/ Institut Pasteur _/ _/ _/ 25 rue du Dr. Roux _/_/_/ _/ _/ F - 75724 PARIS Cedex 15 INSTITUT PASTEUR PARIS France Tel: 33 1 40 61 31 29 Tel: 33 1 45 68 85 38 Fax: 33 1 45 68 86 39 Email: mhaury@pasteur.fr
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